TheIslamic Jihadmilitant group said four of its members were killed in a work accident in the southern Gaza Strip along the border with Israel.
The explosion struck a three-wheeled vehicle near one of the five protest camps Palestinians have set up along the volatile Gaza-Israel border in recent weeks.
Islamic Jihad said all four were killed during preparations without elaborating, implying they were preparing an attack against Israel.
The Israeli military said it was unaware of any activity in the area of the blast.
It comes a day after another mass gathering along the border area in which thousands of Palestinians, some burning Israeli flags and torching tyres, staged a protest against the 10-year-old blockade. Israel has accused Hamas and other militant groups of using the protests as a cover for attacks and says its snipers have only targeted main instigators.
One protester was killed on Friday, bringing the death toll to 28 over the last two weeks, with more than 1,500 wounded by Israeli fire since 30 March.
The marches have been organised by Gazas militant Hamas rulers, but large turnouts on two preceding Fridays were also driven by Gazas dire living conditions and desperation among the territorys two million residents who have been enduring a crippling border closure by Israel and Egypt since 2007.
Rights groups have described the Israeli militarys open-fire regulations as unlawful, saying they permit soldiers to use potentially lethal force against unarmed protesters.
Hamas leaders have sent mixed signals about whether they plan an eventual mass breach of the border fence. The protests are to culminate in a large rally on 15 May, the 70th anniversary of Israels creation.
Palestinians mourn the event as their nakba or catastrophe, when hundreds of thousands were uprooted in the 1948 war over Israels creation.