Czech Republic’s startups pushing innovation forward in 2026!
Following our series of country articles, today we delve into Czechia’s innovation landscape, exploring the factors shaping its startup ecosystem and highlighting the momentum driving its emerging tech companies.
The Czech Republic has historically demonstrated strong capabilities in industrial and engineering-driven technologies. In recent years, the country has gained increasing weight within Central Europe’s startup landscape, particularly in sectors such as cybersecurity, AI, and Fintech. Today, Prague and Brno act as the country’s main innovation hubs, driving much of this momentum. Their growth is supported by leading universities, public institutions such as CzechInvest and the Prague Startup Centre, and regional initiatives, which offer incubation, early-stage funding, and internationalisation support, alongside an active network of local VCs and angel investors.
In this article, we take a closer look at the Czech entrepreneurial landscape by highlighting 10 promising startups, all founded between 2023 and today.
Adalid Sciences: Founded in 2024 in Prague, Adalid Sciences is a BioTech startup developing advanced drug delivery systems for genomic medicine. They focus on lipid nanoparticles that transport and release nucleic acids such as mRNA, siRNA, DNA plasmids, microRNA, and gene-editing tools into human cells. This technology helps tiny medicine packages get safely inside cells so the medicine can work.
Since its founding, the startup has raised a total of €7.9 million in funding. This capital is being used to advance its proprietary lipid nanoparticle platform, expand preclinical validation, and support a partnership-driven business model.
DecisionRules: Headquartered in Prague, this SaaS startup is developing a no-code and low-code business rules engine designed to help organisations manage and automate decision logic without deep technical expertise. The platform allows non-expert users to create, update, and deploy business rules through an intuitive interface, while integrating with existing CRM, ERP, databases, and core systems across sectors such as financial services, insurance, e-commerce, logistics, and healthcare.
Since launching in 2023, DecisionRules has raised €1.6 million to improve the platform, upgrade its cloud infrastructure, and expand its software so businesses can automate decisions at a large scale while keeping the system reliable, secure, and easy to manage as they grow.
Duvo: Launched in 2025, this technology startup is building an agentic automation platform for large organisations, focused on the retail and FMCG sectors. The platform uses autonomous software agents that can increase workflows, make decisions, and execute tasks across multiple enterprise systems without constant human input. Using processes across tools such as ERP, CRM, and supply chain systems, it helps enterprises reduce operational bottlenecks.
Based in Prague, they secured €12.9 million in funding last December. The capital is being used to further develop its agentic architecture and expand enterprise deployments. The funding reflects growing demand for automation solutions that move beyond systems towards more adaptive, decision-driven software.
E2B: This AI and software development startup has a clear objective: to simplify how developers build and run AI-powered applications. To achieve this, it provides a secure, scalable cloud environment. At its core, the platform creates isolated sandbox environments, or virtual machines, that allow AI agents or applications to execute code without risking security or interfering with other systems.
The technology is especially useful for building agentic applications where autonomous AI agents require real runtime environments rather than simple API responses. Headquartered in Prague and founded in 2023, the startup closed a funding round last July, so they have raised to date €29.8 million.
Elin.ai: Addressing the growing impact of online toxicity on mental well-being, this HealthTech startup has developed an AI-powered mobile app designed to support users in challenging digital environments. The platform acts as a personalised cognitive assistant, offering tailored mental resilience advice in real time and adapting its responses as it learns from user interactions.
The AI is developed jointly with psychiatrists, enabling it to emulate professional guidance in situations that may trigger anxiety, stress, or negative thought patterns. Based in Prague, the startup was founded in 2023 and has raised €1 million in funding to date.
Everbot: Bringing together a fragmented AI tooling landscape, this Ostrava-based startup has built a unified platform that aggregates more than 60 leading global AI models into a single, user-friendly interface. Designed for entrepreneurs, marketers, e-shops, and creative professionals, the platform enables users to generate, translate, and manage text, graphics, video, and audio from one dashboard, supported by intelligent automation that streamlines decision-making and content workflows.
Founded in 2024, the startup has raised €972k in funding to support product development and scale its offering, reflecting investor confidence in its approach to simplifying how businesses adopt and use advanced AI technologies.
Finlay: Launched in 2024, this Prague-based AI startup has created a recruitment platform designed to support both candidates and businesses throughout the hiring process. For candidates, it analyses profiles, skills, and career preferences to match them with relevant job opportunities and guide them through applications. For businesses, it processes job requirements, screens and ranks candidates, and identifies the best matches based on skills, experience, and fit.
They secured €300k to grow their position in HR software across Europe by scaling Finlay’s AI, which replaces manual job-board searches and application screening with a faster, smarter way to discover talent, assess fit, and help teams hire with less time, cost, and effort.
Grid.online: Headquartered in Prague, Grid.online develops a shared transportation network for parcel delivery to make urban logistics more efficient. They have built a digital logistics platform that connects businesses needing to ship parcels with drivers or fleets that have unused transport capacity within a city. The system automatically matches delivery requests with nearby vehicles already on the road, assigns parcels, and manages the process through an app and API integrations, enabling on-demand deliveries without the need for companies to use their own fleets.
Founded in 2024, the startup’s core technology functions as an API-driven logistics infrastructure that businesses can plug into, similar to a logistics cloud. By focusing on smarter sharing of existing urban transport capacity to improve efficiency and scalability, they have raised €1.5 million in funding.
Langtail: Founded in 2023, this startup makes it easier for teams to build and manage apps that use AI language models. It provides one central workspace where teams can create, test, and improve the AI prompts and settings that drive their apps, and then deploy them without needing complex engineering work. The platform also has a spreadsheet-style interface that lets teams compare and test different prompts or AI versions, helping them spot problems before the app goes live.
With offices in Prague, Langtail has raised €1 million to date to enhance its platform, supporting real-time monitoring and analytics, centralised logs and metrics, and security features like an AI Firewall that protects against prompt injection and other threats.
VETSTOR: Operating in the pet care market, this Prague-based startup has developed an AI-powered app that connects pet owners and veterinarians through a more personalised and data-driven care app. By building detailed pet health profiles from veterinary appointments, the platform delivers tailored product recommendations, supports appointment booking, and enables direct communication between vets and pet owners, improving access to professional advice and ongoing care.
Since its launch in 2024, the startup secured €1 million in funding to further develop its connected care ecosystem. Its model allows veterinary clinics to run personalised online shops as an additional revenue stream, while integrated tools such as a built-in calendar help veterinarians manage appointments more efficiently and provide more comprehensive, individualised care.
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